Exploring genes involved in benzoic acid biosynthesis in the Populus davidiana transcriptome and their transcriptional activity upon methyl jasmonate treatment.
Identifieur interne : 001396 ( Main/Exploration ); précédent : 001395; suivant : 001397Exploring genes involved in benzoic acid biosynthesis in the Populus davidiana transcriptome and their transcriptional activity upon methyl jasmonate treatment.
Auteurs : Seong-Bum Park [Corée du Sud] ; Jong Youn Kim [Corée du Sud] ; Jung Yeon Han [Corée du Sud] ; Chang-Ho Ahn [Corée du Sud] ; Eung-Jun Park [Corée du Sud] ; Yong Eui Choi [Corée du Sud]Source :
- Journal of chemical ecology [ 1573-1561 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN des plantes (composition chimique), ARN des plantes (isolement et purification), ARN des plantes (métabolisme), Acide benzoïque (composition chimique), Acide benzoïque (métabolisme), Acyl coenzyme A (génétique), Acyl coenzyme A (métabolisme), Acétates (pharmacologie), Aldehyde oxidoreductases (génétique), Aldehyde oxidoreductases (métabolisme), Analyse de séquence d'ARN (MeSH), Cellules végétales (effets des médicaments et des substances chimiques), Cellules végétales (métabolisme), Cyclopentanes (pharmacologie), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Oxylipines (pharmacologie), Phenylalanine ammonia-lyase (génétique), Phenylalanine ammonia-lyase (métabolisme), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Réaction de polymérisation en chaine en temps réel (MeSH), Transcriptome (effets des médicaments et des substances chimiques).
- MESH :
- composition chimique : ARN des plantes, Acide benzoïque.
- effets des médicaments et des substances chimiques : Cellules végétales, Transcriptome.
- génétique : Acyl coenzyme A, Aldehyde oxidoreductases, Feuilles de plante, Phenylalanine ammonia-lyase, Populus, Protéines végétales.
- isolement et purification : ARN des plantes.
- métabolisme : ARN des plantes, Acide benzoïque, Acyl coenzyme A, Aldehyde oxidoreductases, Cellules végétales, Feuilles de plante, Phenylalanine ammonia-lyase, Populus, Protéines végétales.
- pharmacologie : Acétates, Cyclopentanes, Oxylipines.
- Analyse de séquence d'ARN, Réaction de polymérisation en chaine en temps réel.
English descriptors
- KwdEn :
- Acetates (pharmacology), Acyl Coenzyme A (genetics), Acyl Coenzyme A (metabolism), Aldehyde Oxidoreductases (genetics), Aldehyde Oxidoreductases (metabolism), Benzoic Acid (chemistry), Benzoic Acid (metabolism), Cyclopentanes (pharmacology), Oxylipins (pharmacology), Phenylalanine Ammonia-Lyase (genetics), Phenylalanine Ammonia-Lyase (metabolism), Plant Cells (drug effects), Plant Cells (metabolism), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Populus (metabolism), RNA, Plant (chemistry), RNA, Plant (isolation & purification), RNA, Plant (metabolism), Real-Time Polymerase Chain Reaction (MeSH), Sequence Analysis, RNA (MeSH), Transcriptome (drug effects).
- MESH :
- chemical , chemistry : Benzoic Acid, RNA, Plant.
- chemical , genetics : Acyl Coenzyme A, Aldehyde Oxidoreductases, Phenylalanine Ammonia-Lyase, Plant Proteins.
- chemical , isolation & purification : RNA, Plant.
- chemical , metabolism : Acyl Coenzyme A, Aldehyde Oxidoreductases, Benzoic Acid, Phenylalanine Ammonia-Lyase, Plant Proteins, RNA, Plant.
- chemical , pharmacology : Acetates, Cyclopentanes, Oxylipins.
- drug effects : Plant Cells, Transcriptome.
- genetics : Plant Leaves, Populus.
- metabolism : Plant Cells, Plant Leaves, Populus.
- Real-Time Polymerase Chain Reaction, Sequence Analysis, RNA.
Abstract
Benzoic acids (BAs) are important structural elements in a wide variety of essential compounds and natural products, and play crucial roles in plant fitness. BA is a precursor of diverse benzenoid compounds, including the hormone salicylic acid (SA) and the aglycone moiety of salicin, which is particularly important in the Salicaceae family. The biosynthetic pathways leading to BA formation in plants are largely unknown. Recently, the CoA-dependent β-oxidative BA biosynthesis pathway, which occurs in peroxisomes, has been characterized in petunia. The core of this pathway is cinnamic acid → cinnamoyl-CoA → 3-hydroxy-3-phenylpropanoyl-CoA → 3-oxo-3-phenylpropanoyl-CoA → benzoyl-CoA. Here, we used 454 pyrosequencing to analyze the transcriptome of Populus davidiana and isolate putative genes involved in BA biosynthesis. De novo assembly generated 57,322 unique sequences, including 15,217 contigs and 42,105 singletons. From the unique sequences, we selected six genes exhibiting high similarity to genes encoding L-phenylalanine ammonia lyase, cinnamate:CoA ligase, cinnamoyl-CoA hydratase-dehydrogenase, 3-ketoacyl-CoA thiolase, benzoyl-CoA:benzyl alcohol O-benzoyltransferase, and benzaldehyde dehydrogenase. Each of these enzymes might be involved in BA biosynthesis. Real-time PCR (qPCR) analysis revealed that these six genes were highly transcribed in the aerial organs of P. davidiana, particularly in leaves. Treating the leaves of in vitro cultured plants with methyl jasmonate (MeJA) strongly enhanced the mRNA accumulation of all 6 genes, and this treatment also clearly enhanced the accumulation of BA, SA, salicyl alcohol, benzyl alcohol, benzyl benzoate, and benzaldehyde but not salicin. Our study shows that P. davidiana may possess a CoA-dependent β-oxidative BA synthesis pathway. We also identified a relationship between the transcription of these genes and the accumulation of benzenoids, including BA and SA, which are highly responsive to the defense signaling molecule (MeJA).
DOI: 10.1007/s10886-017-0903-3
PubMed: 29129016
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Sud</country><wicri:regionArea>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701</wicri:regionArea><wicri:noRegion>200-701</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Jong Youn" sort="Kim, Jong Youn" uniqKey="Kim J" first="Jong Youn" last="Kim">Jong Youn Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701</wicri:regionArea><wicri:noRegion>200-701</wicri:noRegion></affiliation></author><author><name sortKey="Han, Jung Yeon" sort="Han, Jung Yeon" uniqKey="Han J" first="Jung Yeon" last="Han">Jung Yeon Han</name><affiliation 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Sud</country><wicri:regionArea>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701</wicri:regionArea><wicri:noRegion>200-701</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of chemical ecology</title><idno type="eISSN">1573-1561</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetates (pharmacology)</term><term>Acyl Coenzyme A (genetics)</term><term>Acyl Coenzyme A (metabolism)</term><term>Aldehyde Oxidoreductases (genetics)</term><term>Aldehyde Oxidoreductases (metabolism)</term><term>Benzoic Acid (chemistry)</term><term>Benzoic Acid (metabolism)</term><term>Cyclopentanes (pharmacology)</term><term>Oxylipins (pharmacology)</term><term>Phenylalanine Ammonia-Lyase (genetics)</term><term>Phenylalanine Ammonia-Lyase (metabolism)</term><term>Plant Cells (drug effects)</term><term>Plant Cells (metabolism)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>RNA, Plant (chemistry)</term><term>RNA, Plant (isolation & purification)</term><term>RNA, Plant (metabolism)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Sequence Analysis, RNA (MeSH)</term><term>Transcriptome (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (composition chimique)</term><term>ARN des plantes (isolement et purification)</term><term>ARN des plantes (métabolisme)</term><term>Acide benzoïque (composition chimique)</term><term>Acide benzoïque (métabolisme)</term><term>Acyl coenzyme A (génétique)</term><term>Acyl coenzyme A (métabolisme)</term><term>Acétates (pharmacologie)</term><term>Aldehyde oxidoreductases (génétique)</term><term>Aldehyde oxidoreductases (métabolisme)</term><term>Analyse de séquence d'ARN (MeSH)</term><term>Cellules végétales (effets des médicaments et des substances chimiques)</term><term>Cellules végétales (métabolisme)</term><term>Cyclopentanes (pharmacologie)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Oxylipines (pharmacologie)</term><term>Phenylalanine ammonia-lyase (génétique)</term><term>Phenylalanine ammonia-lyase (métabolisme)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Réaction de polymérisation en chaine en temps réel (MeSH)</term><term>Transcriptome (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzoic Acid</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Acyl Coenzyme A</term><term>Aldehyde Oxidoreductases</term><term>Phenylalanine Ammonia-Lyase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acyl Coenzyme A</term><term>Aldehyde Oxidoreductases</term><term>Benzoic Acid</term><term>Phenylalanine Ammonia-Lyase</term><term>Plant Proteins</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acetates</term><term>Cyclopentanes</term><term>Oxylipins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ARN des plantes</term><term>Acide benzoïque</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Plant Cells</term><term>Transcriptome</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Cellules végétales</term><term>Transcriptome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Acyl coenzyme A</term><term>Aldehyde oxidoreductases</term><term>Feuilles de plante</term><term>Phenylalanine ammonia-lyase</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN des plantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Cells</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term><term>Acide benzoïque</term><term>Acyl coenzyme A</term><term>Aldehyde oxidoreductases</term><term>Cellules végétales</term><term>Feuilles de plante</term><term>Phenylalanine ammonia-lyase</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acétates</term><term>Cyclopentanes</term><term>Oxylipines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term><term>Sequence Analysis, RNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ARN</term><term>Réaction de polymérisation en chaine en temps réel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Benzoic acids (BAs) are important structural elements in a wide variety of essential compounds and natural products, and play crucial roles in plant fitness. BA is a precursor of diverse benzenoid compounds, including the hormone salicylic acid (SA) and the aglycone moiety of salicin, which is particularly important in the Salicaceae family. The biosynthetic pathways leading to BA formation in plants are largely unknown. Recently, the CoA-dependent β-oxidative BA biosynthesis pathway, which occurs in peroxisomes, has been characterized in petunia. The core of this pathway is cinnamic acid → cinnamoyl-CoA → 3-hydroxy-3-phenylpropanoyl-CoA → 3-oxo-3-phenylpropanoyl-CoA → benzoyl-CoA. Here, we used 454 pyrosequencing to analyze the transcriptome of Populus davidiana and isolate putative genes involved in BA biosynthesis. De novo assembly generated 57,322 unique sequences, including 15,217 contigs and 42,105 singletons. From the unique sequences, we selected six genes exhibiting high similarity to genes encoding L-phenylalanine ammonia lyase, cinnamate:CoA ligase, cinnamoyl-CoA hydratase-dehydrogenase, 3-ketoacyl-CoA thiolase, benzoyl-CoA:benzyl alcohol O-benzoyltransferase, and benzaldehyde dehydrogenase. Each of these enzymes might be involved in BA biosynthesis. Real-time PCR (qPCR) analysis revealed that these six genes were highly transcribed in the aerial organs of P. davidiana, particularly in leaves. Treating the leaves of in vitro cultured plants with methyl jasmonate (MeJA) strongly enhanced the mRNA accumulation of all 6 genes, and this treatment also clearly enhanced the accumulation of BA, SA, salicyl alcohol, benzyl alcohol, benzyl benzoate, and benzaldehyde but not salicin. Our study shows that P. davidiana may possess a CoA-dependent β-oxidative BA synthesis pathway. We also identified a relationship between the transcription of these genes and the accumulation of benzenoids, including BA and SA, which are highly responsive to the defense signaling molecule (MeJA).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29129016</PMID><DateCompleted><Year>2018</Year><Month>01</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-1561</ISSN><JournalIssue CitedMedium="Internet"><Volume>43</Volume><Issue>11-12</Issue><PubDate><Year>2017</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Journal of chemical ecology</Title><ISOAbbreviation>J Chem Ecol</ISOAbbreviation></Journal><ArticleTitle>Exploring genes involved in benzoic acid biosynthesis in the Populus davidiana transcriptome and their transcriptional activity upon methyl jasmonate treatment.</ArticleTitle><Pagination><MedlinePgn>1097-1108</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10886-017-0903-3</ELocationID><Abstract><AbstractText>Benzoic acids (BAs) are important structural elements in a wide variety of essential compounds and natural products, and play crucial roles in plant fitness. BA is a precursor of diverse benzenoid compounds, including the hormone salicylic acid (SA) and the aglycone moiety of salicin, which is particularly important in the Salicaceae family. The biosynthetic pathways leading to BA formation in plants are largely unknown. Recently, the CoA-dependent β-oxidative BA biosynthesis pathway, which occurs in peroxisomes, has been characterized in petunia. The core of this pathway is cinnamic acid → cinnamoyl-CoA → 3-hydroxy-3-phenylpropanoyl-CoA → 3-oxo-3-phenylpropanoyl-CoA → benzoyl-CoA. Here, we used 454 pyrosequencing to analyze the transcriptome of Populus davidiana and isolate putative genes involved in BA biosynthesis. De novo assembly generated 57,322 unique sequences, including 15,217 contigs and 42,105 singletons. From the unique sequences, we selected six genes exhibiting high similarity to genes encoding L-phenylalanine ammonia lyase, cinnamate:CoA ligase, cinnamoyl-CoA hydratase-dehydrogenase, 3-ketoacyl-CoA thiolase, benzoyl-CoA:benzyl alcohol O-benzoyltransferase, and benzaldehyde dehydrogenase. Each of these enzymes might be involved in BA biosynthesis. Real-time PCR (qPCR) analysis revealed that these six genes were highly transcribed in the aerial organs of P. davidiana, particularly in leaves. Treating the leaves of in vitro cultured plants with methyl jasmonate (MeJA) strongly enhanced the mRNA accumulation of all 6 genes, and this treatment also clearly enhanced the accumulation of BA, SA, salicyl alcohol, benzyl alcohol, benzyl benzoate, and benzaldehyde but not salicin. Our study shows that P. davidiana may possess a CoA-dependent β-oxidative BA synthesis pathway. We also identified a relationship between the transcription of these genes and the accumulation of benzenoids, including BA and SA, which are highly responsive to the defense signaling molecule (MeJA).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Seong-Bum</ForeName><Initials>SB</Initials><AffiliationInfo><Affiliation>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jong Youn</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Jung Yeon</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahn</LastName><ForeName>Chang-Ho</ForeName><Initials>CH</Initials><AffiliationInfo><Affiliation>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Eung-Jun</ForeName><Initials>EJ</Initials><AffiliationInfo><Affiliation>Division of Forest Biotechnology, Korea Forest Research Institute, Suwon, 441-847, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Yong Eui</ForeName><Initials>YE</Initials><AffiliationInfo><Affiliation>Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 200-701, South Korea. yechoi@kangwon.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>S111414L070110</GrantID><Agency>Kangwon National University</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>11</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Chem Ecol</MedlineTA><NlmUniqueID>7505563</NlmUniqueID><ISSNLinking>0098-0331</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000085">Acetates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000214">Acyl Coenzyme A</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003517">Cyclopentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054883">Oxylipins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>19774-86-8</RegistryNumber><NameOfSubstance UI="C049446">3-ketohexanoyl-coenzyme A</NameOfSubstance></Chemical><Chemical><RegistryNumber>8SKN0B0MIM</RegistryNumber><NameOfSubstance UI="D019817">Benzoic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>900N171A0F</RegistryNumber><NameOfSubstance UI="C072239">methyl jasmonate</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.2.-</RegistryNumber><NameOfSubstance UI="D000445">Aldehyde Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.2.1.28</RegistryNumber><NameOfSubstance UI="C027015">benzaldehyde dehydrogenase (NAD+)</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.3.1.24</RegistryNumber><NameOfSubstance UI="D010650">Phenylalanine Ammonia-Lyase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000085" MajorTopicYN="N">Acetates</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000214" MajorTopicYN="N">Acyl Coenzyme A</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000445" MajorTopicYN="N">Aldehyde Oxidoreductases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019817" MajorTopicYN="N">Benzoic Acid</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003517" MajorTopicYN="N">Cyclopentanes</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054883" MajorTopicYN="N">Oxylipins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010650" MajorTopicYN="N">Phenylalanine Ammonia-Lyase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Benzoic acid biosynthesis</Keyword><Keyword MajorTopicYN="N">Populus davidiana</Keyword><Keyword MajorTopicYN="N">Transcriptome analysis</Keyword><Keyword MajorTopicYN="N">de novo sequencing</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>07</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>11</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>10</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>11</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>11</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29129016</ArticleId><ArticleId IdType="doi">10.1007/s10886-017-0903-3</ArticleId><ArticleId 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Eung Jun" sort="Park, Eung Jun" uniqKey="Park E" first="Eung-Jun" last="Park">Eung-Jun Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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